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B016448 - GENERAL AND APPLIED BIOCHEMISTRY
Main information
Teaching Language
Course Content
Suggested readings
Learning Objectives
Prerequisites
Teaching Methods
Further information
Type of Assessment
Course program
Academic Year 2019-20
Coorte 2018 - 3-years First Cycle Degree (DM 270/04) in APPLIED PHARMACEUTICAL SCIENCES- QUALITY CONTROL AND ASSURANCE
Course year
Second year - First Semester
Belonging Department
Neurosciences, Psychology, Drug Research and Child Health (NEUROFARBA)
Course Type
Single education field course
Scientific Area
BIO/10 - BIOCHEMISTRY
Credits
9
Teaching Hours
72
Teaching Term
23/09/2019 ⇒ 11/01/2020
Attendance required
No
Type of Evaluation
Final Grade
Course Content
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Course program
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Lectureship
Teaching Language
Italian
Course Content
Structure and function of proteins. Principles of thermodynamics. Kinetic and enzymatic catalysis. Structure and function of carbohydrates, lipids and nucleic acids. The metabolism of proteins, carbohydrates, lipids and nucleic acids. Spectrophotometric and Electrophoretic techniques.
Suggested readings (Search our library's catalogue)
Garrett R.H., Grisham C.M., “BIOCHIMICA”, Ed. Piccin
Nelson D.L., Cox M.M. “I PRINCIPI DI BIOCHIMICA DI LEHNINGER”, Ed. Zanichelli
Berg J.M. , Tymoczko J.L., Stryer L. “BIOCHIMICA”, Ed. Zanichelli
Nelson D.L., Cox M.M. “I PRINCIPI DI BIOCHIMICA DI LEHNINGER”, Ed. Zanichelli
Berg J.M. , Tymoczko J.L., Stryer L. “BIOCHIMICA”, Ed. Zanichelli
Learning Objectives
The course aims to train the student in relation to the general principles of Biochemistry. This course provides knowledge about the structure and function of biological macromolecules, methods and techniques used for their study, metabolism and metabolic regulation.
Prerequisites
The course requires knowledge of general chemistry, organic and basic notions of biology.
Teaching Methods
Lectures
Further information
student reception hours
Prof. P. Cirri: tuesday 14,00-17,00
Prof.ssa D. Degl'Innocenti: monday 15,30
Location:
Dipartimento di Scienze Biomediche Sperimentali e Cliniche "Mario Serio", viale Morgagni 50, 50134 Firenze.
Prof. P. Cirri: tuesday 14,00-17,00
Prof.ssa D. Degl'Innocenti: monday 15,30
Location:
Dipartimento di Scienze Biomediche Sperimentali e Cliniche "Mario Serio", viale Morgagni 50, 50134 Firenze.
Type of Assessment
Oral examination
Course program
General principles of biochemistry. The amino acids. The peptide bond. Structure of proteins. Hemoglobin. Enzymes and enzyme kinetics. Regulation of enzyme activity. Inhibition of enzyme activity. Mechanism of enzyme action. Allosteric enzymes. Biological macromolecules: lipids and lipoproteins, carbohydrates and glycoproteins, nucleic acids. General principles of metabolism. Glycolysis. Pentose-phosphate cycle. Citric acid cycle. Respiratory chain and oxidative phosphorylation. Glycogen synthesis and glycogen-lysis. Mechanisms of hormonal regulation of glucose metabolism. Activation, transport and oxidation of fatty acids. Chetonic bodies. Biosynthesis of fatty acids. Cholesterol metabolism. Mechanisms of hormonal regulation of lipid metabolism. Degradation of amino acids: urea cycle and fate of carbon skeleton. General aspects of pyrimidine and purine bases metabolism. Molecules derived from amino acids. DNA duplication. Transcription and translation. Integration and hormonal regulation of metabolism in mammals.
Applied Biochemistry: spectrophotometry: principles and practical applications. assay methods of the enzymes. Determination of the main kinetic parameters. Electrophoretic techniques. Analysis of protein profiles. Mass Spectrometry.
Applied Biochemistry: spectrophotometry: principles and practical applications. assay methods of the enzymes. Determination of the main kinetic parameters. Electrophoretic techniques. Analysis of protein profiles. Mass Spectrometry.