The course devoted to the in-depth study of drug analysis using chromatographic techniques coupled with mass spectrometry. Complex experiments in mass spectrometry and accurate mass measurements will be introduced. Finally, practice lessons will be carried out on the theoretical concepts developed during the lessons.
Introduction of Mass Spectrometry J. Throck Watson
Learning Objectives
To introduce the student to modern and advanced analysis techniques in the pharmaceutical field using mass spectrometry as an instrumental system of analysis.
The aim of the couse is to show the student the modern and advanced analytical techniques in the pharmaceutical field using mass spectrometry as instrumental system of analysis. The course is divided into theoretical lessons and practical applications, to which a lot of time is dedicated. At the end of the course the student will have developed:
- the ability to choose, distinguish and select the best instrumental configuration to apply to the proposed analysis;
- separation and quantification of interest pharmaceutical substances, based on their chemical characteristics.
- the ability to process, elaborate, explain and evaluate the meaning of the results obtained experimentally;
- awareness of maintenance, safety risks, purchase and maintenance costs associated with the equipment used.
Prerequisites
Attendance at the course is mandatory (Directive 85/432 / EEC); the minimum frequency required is 90% (Regulations of the CdS). In order to attend the course it is necessary to have attended the courses of the first two years of the in Applied Pharmaceutical Sciences degree course.
For the final examination for this course, the student must have successfully passed General Chemistry, Organic Chemistry, Drug Analysis I-II-III and IV examinations. It is also recommended the Analytical Chemistry skills.
Teaching Methods
The course will be divided into theoretical lessons (3 CFU) and exercises (3 CFU). The theoretical lesson will anticipate the exercise lessons, allowing the instructions for their solve.
The teaching material and some audio recordings explaining the activities will be uploaded to the Moodle e-learning platform (https://e-l.unifi.it) available to students. Lessons and exercises will carry out via video projection.
Further information
Office hours: every day, by appointment Department of NEUROFARBA, Section of Pharmaceuticals and Nutraceuticals
Via U. Schiff, 6 - 50019 Sesto F.no - Florence
e-mail: gianluca.bartolucci@unifi.it
Type of Assessment
The examination consists of a test (about 60 minutes) which focuses on all the topics covered during the lessons: theoretical and pratical aspects. The test can be carried out during any exam session shown on the University Exam Booking Service page, subject to compliance with the prerequisites, and consists of various types of questions: open answer / multiple choice / problem solving. In the test, the student demonstrates the knowledge and understanding acquired by applying them to the resolution of exercises (about 35% of which contributes to the formation of the grade) and the ability to process and communicate the knowledge and tools acquired during the course ( 35%) using appropriate scientific language (30%). The final results from all these contributions considered important indicators of the adequacy of the level of learning achieved and of the achievement of the results envisaged by the course.
Course program
Mass spectrometry theory and operation. Mass spectrometer, vacuum system, ion sources, mass analyzers. Accurate mass measurements. Tandem mass spectrometry.